TABLE 5.
Kinetics of cleavage of Ac-KSGVFVVNGK-NH2 with HIV-1 PR and FIV PRa
PR type | Mean Km (μM) ± SD | Mean kcat (s−1) ± SD | Mean kcat/Km (μM−1 s−1) (10−4) ± SD |
---|---|---|---|
FIV | 2,100 ± 300 | 0.70 ± 0.08 | 3.4 ± 0.8 |
HIV-1 | 420 ± 40 | 0.35 ± 0.02 | 8 ± 1 |
Peptide cleavage kinetics of HIV-1 PR and FIV PR cleavage of Ac-KSGVF↓VVNGK-NH2 at pH 6.7 in 0.2 M NaCl and 0.1 M MES are shown. Errors are shown as 1 standard deviation of the data from three trials.