TABLE 7.
Summary of phage and peptide substratesa
Substrate | HIV-1 PR only | FIV PR only | Both HIV-1 PR and FIV PR | Neither PRc |
---|---|---|---|---|
Phage | GSGIF↓LETSL | GALTN↓AVLVP | GSGIY↓TVQSL | |
GSGVF↓VETSL | SGRIN↓VALVP | GSGVF↓VVNGL | ||
GSGVF↓VEMPL | GAMVN↓QALVP | GSGVY↓HVSTL | ||
GSGIM↓FESNL | SGVAL/SALVP | GSGVF↓AVTQL | ||
GSGLF↓TEYGL | GSGVF/YTLVP | |||
GSGVH↓VEYTL | GSGVY↓QL↓SAL | |||
GSGNY↓FVETL | ||||
GSGNY↓FVQGL | ||||
GSGNY↓LVTSL | ||||
VSQNY↓PIVQL | ||||
GSGVY↓LATDL | ||||
Peptideb | KSGNF↓VVNGLVK | KSGNFVVN↓GLVK | KSGVF↓VVNGLVK | KSGVN / VVNGK |
KSGVF↓VENGLVK | KSGVF↓SVNGK | KSFVF↓VVNGLVK | KSGVQ / VVNGK | |
KSGVF↓VQNGLVK | KSGVFHVN↓GLVK | KSGIF↓VVNGLVK | KSGVF / VNNGLVK | |
KSGVFQVN↓GLVK | KSGVF↓HVNGK | |||
KSGVF↓QVNGLVK | ||||
KSGVF↓VVQGLVK | ||||
KSGVF↓VVTGLVK |
Phage substrates were divided into the categories listed above based on an analysis of Fig. 1.
Peptide substrates were only listed in columns if at least a 100 μM concentration of the substrate was cleaved better than 30% under the conditions listed in Materials and Methods and in Table 2.
Products of the cleavage of substrates listed in the neither PR column were not detected at all.