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. 2024 Oct 4;10(40):eadn8760. doi: 10.1126/sciadv.adn8760

Fig. 1. TRAIL and TRAIL-R2 expression and interaction are augmented in hypoxia.

Fig. 1.

(A) Trail, Trail-R1, and Trail-R2 mRNA expression in HMEC-1 after exposure to hypoxia (2% O2) or normoxia (21% O2) for 24 hours, qPCR normalized to β-actin (n = 6 to 8 per treatment). (B) Total internal reflection fluorescence microscopy images (left) and quantification (right) of TRAIL cell surface expression (n = 3 per treatment). (C) TRAIL-R2 cell surface expression by flow cytometry (n = 4 per treatment). (D) Left: Western blot of TRAIL-R1 and β-actin. Right: TRAIL-R1 protein expression normalized to β-actin (n = 4 per group). (E) Protein-protein interactions between TRAIL–TRAIL-R1 and TRAIL–TRAIL-R2, measured using the Duolink Proximity Ligation Assay. Interactions are indicated by red staining. Left: Representative image. Right: Quantification (n = 6 per treatment). Results are means ± SEM; Students t test or two-way analysis of variance (ANOVA); *P < 0.05, **P < 0.01, and ***P < 0.001.