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. 2024 Oct 4;10(40):eadn8760. doi: 10.1126/sciadv.adn8760

Fig. 6. TRAIL-receptor activation stimulates features of neo-angiogenesis.

Fig. 6.

(A) Trail+/+ and Trail−/− EC proliferation is increased with MD5-1 at 24 hours, normalized to immunoglobulin G (IgG; n = 4 to 5 per group). (B) Representative images showing increased tubule formation of Trail+/+ ECs with MD5-1 versus IgG at 6 hours (10 ng/ml); scale bars, 500 μm. (C) Aortic rings from wild-type mice were exposed to MD5-1 or IgG for 4 days, and sprout number and total sprout length were assessed (n = 3 to 4 per group). (D) Aortic rings from Trail-R−/− mice exposed to hypoxia for 6 days have reduced sprout number and reduced total sprout length (n = 4 per group). Human TRAIL-R2 agonistic antibody (αTRAIL-R2, 10 ng/ml) stimulates HMEC-1 (E) proliferation, (F) migration, and (G) tubule formation (n = 5 to 7 per group). Conversely, neutralization by αTRAIL-R1 has no effect on TRAIL-inducible HMEC-1 (H) proliferation and (I) migration (n = 3 to 4 per group). Results are means ± SEM; two-way ANOVA, Mann-Whitney U test, or Students t test; *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.