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. 2024 Oct 4;10(40):eado3514. doi: 10.1126/sciadv.ado3514

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Fig. 2. — (A) D1 and D2 MSN chromatin accessibility around TSSs was compared across treatment groups using ATAC-seq. SS (control saline), SC (acute cocaine in drug-naïve mice), CS (withdrawal from chronic cocaine), and CC (cocaine challenge following withdrawal from chronic cocaine) (see Fig. 1A). (B) Genome-browser view of the Fosb gene locus showing increased accessibility in D1 MSNs following cocaine exposure, both over its promoter and a putative enhancer region upstream. (C) Differentially accessible genomic loci after prolonged withdrawal from chronic cocaine (CS), showing predominant opening (up) of chromatin in D1 MSNs. (D) Motif analysis on enhancer regions showed increased accessibility specifically in D1 MSNs after withdrawal (Homer de novo motif analysis). (E) Heatmap of differentially accessible enhancers between D1 and D2 MSNs, showing corresponding D1 versus D2 MSN expression of the most proximal genes. The heatmap below shows corresponding gene expression in D1 MSNs with acute cocaine (SC), withdrawal (CS), and cocaine challenge (CC) compared to saline control (SS).