Table 2:
Details of primary antibodies used in the study.
| Reagent | Dilution | Application | Supplier | Catalog number |
|---|---|---|---|---|
|
| ||||
| 4’,6-Diamidino-2-Phenylindole, Dihydrochloride (DAPI) | 1:2000 | IF | Life technologies | D1306 |
| Sir-Actin | 1: 1000 | IF | Tebu-Bio | SC001 |
| Mouse anti-β1 integrin | 1:1000 | WB | BD Biosciences | 610468 |
| Mouse anti-α3 integrin (ASC-1) | 1:100 | IF | Abcam | ab228425 |
| Rabbit anti-α3 integrin | 1:1000 | MP | Abcam | ab131055 |
| Rabbit anti-α3 integrin | 1:1000 | WB | Abcam | ab131055 |
| Mouse anti-β4 integrin | 1:100 | IF, WB | Millipore | MAB1964 |
| Rat anti-β4 integrin | 1:100 | MP | Abcam | ab95583 |
| Rat anti-α6 integrin (CD49f, cloneGoH3) | 1:100 | IF | Serotec | MCA699 |
| Rat anti-α6 integrin | 1:500 | MP | Novus | 85747 |
| Rabbit anti-α6 integrin | 1:1000 | WB | Abcam | ab97760 |
| Rabbit anti-β-catenin (E247) | 1:100 | IF | Abcam | ab32572 |
| Mouse anti-β-catenin | 1:500 | MP | Cell Marque | 224M-14 |
| Mouse anti-CD151 | 1:100 | IF | Abcam | ab33315 |
| Rabbit anti-phospho-MLC 2 (Thr18/Ser19) | 1:100, 1:1000, 1:1000 | IF, MP, WB | Cell Signaling Technology | 3674 |
| Rabbit anti-COLXVII (EPR18614) | 1:100, 1:500, 1:1000 | IF, MP, WB | Abcam | ab184996 |
| Mouse anti-vinculin | 1:100, 1:1000 | IF, WB | Sigma | V9131 |
| Rat anti-Hsc70/Hsp73 | 1:1000 | WB | Enzo | ADI-SPA-815 |
| Guinea pig anti-keratin 14 | 1: 100, 1:1000 | IF, WB | Covance | PRB-155P |
| Guinea pig anti-keratin 14 | 1: 1000 | MP | Progen | GP-CK14 |
| Mouse anti-pan cytokeratin | 1:150 | MP | Abcam | ab7753 |
| Mouse anti-pan cytokeratin | 1: 100 | MP | Invitrogen | MA5 13156 |
| Rabbit anti-Fibronectin | 1:1000, 1:1000 | MP, WB | Sigma | F3648 |
| Rabbit anti-Collagen I | 1:1000 | MP | Novus | NB600–408 |
| Rabbit anti-pan-laminin | 1:100 | MP | Sigma | L9393 |
| Mouse anti-E-cadherin | 1:200 | MP | BD Biosciences | 610182 |
| Mouse anti-α-SMA | 1:2000 | MP | DAKO | M0851 |
| Rabbit anti-AMOTL2 | 1:100, 1:1000 | IF, WB | Proteintech | 23351–1-AP |
| Mouse anti-YAP | 1:100, 1:50 | IF, MP | Santa Cruz | sc-101199 |
IF= immunofluorescence, MP= multiplex fluorescence immunohistochemistry, WB= western blot.