TABLE 3.
Primers used for quantitative RT-PCR
| Gene/description | Primer sequence 5’−3’ | PCR efficencya | Reference |
|---|---|---|---|
| stx1/Shiga toxin type I | |||
| GACTGCAAAGACGTATGTAGATTCG | 94% | This publication | |
| TCAATCATCAGTAAAGACGTACCTCC | |||
| stx2/Shiga toxin type II | |||
| ATTAACCACACCCCACCGG | 96% | This publication | |
| TGCCGTATTAACGAACCCG | |||
| iha/Bifunctional siderophore receptor/adhesin | |||
| GCTGGCTATGATCATACTTTC | 102% | This publication | |
| AGCCCCCATTTGTCGCGC | |||
| eae/Intimin | |||
| GCTTACTATTACCGTTCTGT | 93% | This publication | |
| CGTTTTGGCACTATTTGCCC | |||
| toxB/Toxin B | |||
| ATATCGTAAGTAACTCAGGAAAC | 89% | This publication | |
| GTATCCGTCGATGTTATTACTTG | |||
| ehxA/Enterohaemolysin | |||
| AGATGCAGCCCTGACAACAA | 95% | This publication | |
| CCGGTTAATGCACTCACCAG | |||
a
PCR efficiency represents the average for the primer set across the experiments. PCR efficacy was determined from a 5 log 5-point standard curve using pooled cDNA template.