Figure 5. Enrichment of Lithium modulated genes in synaptic structures in Layer 2/3 excitatory neurons from wildtype and DKOs.

(A) SynGO plot of enrichment of gene sets associated with synaptic structures in wt (left) and DKOs (right). Significantly associated genes are found in the top-layered pre- and postsynapse and branch our most prominently into post-synaptic membrane associated structures. (B) Protein network of lithium deregulated genes forms a highly interconnected cluster (Up- and downregulated gene products are depicted as blue and red circled nodes, respectively. Protein-protein-interactions (PPI) are plotted as greyish lines between nodes. Nodes added by the PPI network to increase connectivity are plotted as smaller sized grey circles. Only 4 genes are not integrated into the main network based on published PPIs (C-E) Synaptic components from the SynGO pre-synapse (C), post-synapse (D) and post-synaptic density (E) subclusters are highlighted in yellow and are enriched at the ‘synaptic core’ of the network.