| Commercially available reagents: | ||
|---|---|---|
| Probe | Cat.# | Source: |
| CenSat D14Z1/D22Z1 | LPE 014 | Cytocell |
| CenSat D15Z4 | LPE 015 | Cytocell |
| CenSat D7Z1 | LPE 07 | Cytocell |
| CenSat D8Z2 | LPE 08 | Cytocell |
| CenSat D12Z3 | LPE 012 | Cytocell |
| CenSat D20Z1 | LPE 020 | Cytocell |
| CenSat DXZ1 | LPE 0X | Cytocell |
| Chr.22 qter | LPT 22Q | Cytocell |
| Human 45S rDNA probe | BAC RP11-450E20 | Empire Genomics |
| Human 5S rDNA probe | WI2 1774C11, WI2 2801N10 | Empire Genomics |
| Chr.14 near-cen marker | RP11-55G7 | Empire Genomics |
| Chr.21 near-cen marker | BAC RP11-846C20 | Empire Genomics |
| Chr.22 near-cen marker | BAC RP11-806D22 | Empire Genomics |
| Antibody | ||
| Anti-UBF | NBP1-82545 | Novus Biologicals |
| Anti-Nucleolin | ab70493 | Abcam |
| Anti-Treacle/TCOF1 | ab224544 | Abcam |
Secondary antibodies for immunofluorescence (AlexaFluor 647 conjugates) were obtained from Life Technologies and used at 1:500 dilution.