Skip to main content
. 2024 Oct 4;13:RP94849. doi: 10.7554/eLife.94849

Figure 2. Epithelial OGT deletion inhibits mouse colorectal tumorigenesis.

(A) Western blot analysis of OGT expression in intestinal tissues and counting tumor numbers in APCmin and Ogt IEC cKO mice. (B) Histology analysis of intestinal carcinogenesis by HE staining, scale bar: up panel 275 μm, bottom panel 20 μm, n=3, respectively. (C) Real-time PCR analysis of cytokines mRNA expression in intestine. (D) ELISA analysis of cytokines expression in intestine. Statistical significance was determined by unpaired Student’s t-test, *p<0.05, **p<0.01, ***p<0.001, ns, no significant difference. Data represent the mean of ± SD.

Figure 2.

Figure 2—figure supplement 1. The genotype of APCmin,Villin-Cre and Ogtfl/fl mice.

Figure 2—figure supplement 1.

Genomic DNA was extracted from the tails of APCmin, Villin-Cre, and Ogtfl/fl mice and used for PCR with various primers. The resulting products were separated by agarose gel electrophoresis to determine the genotype.