Figure 8. The MSTN^Del73C mutation with FGF5 knockout inhibit calcium-dependent transcription signal pathway.

(A–B) The protein expression level of CaMKII α/δ between WT and MF^+/- cells at GM (n=3). (C) Distribution of intracellular Ca²⁺ signals between WT and MF^+/- cells at GM. (D) Average intracellular Ca²⁺ fluorescence intensity between WT and MF^+/- cells at GM (n=4). (E) The mRNA expression levels of Ca²⁺ channels and myoblast fusion-related genes at GM (n=3). (F) The mRNA expression levels of Ca²⁺ channels and myoblast fusion-related genes at DM2 (n=3). Data: mean ± SEM. Unpaired student’s t-test was used for statistical analysis. All student’s t-test were performed after the equal variance test, otherwise the t-test with Welch’s correction were used. *p<0.05, **p<0.01.