FIG. 1.

CRSV genome, location of CP amino acid polymorphisms, and corresponding assembly and systemic movement phenotypes. (A) Genome organization. RNA-1 contains ORFs (denoted by open boxes) for p27 and the CP. Occasionally, translation of p27 is extended by a −1 ribosomal frameshift (FS) event to produce p88. RNA-2 codes for the MP. (B) CRSV RNA-1 constructs. Schematic diagram displaying the relative positions of amino acid polymorphisms in the 339-amino acid CP (denoted by numbers below the CP ORF). Locations of restriction sites used for the construction and differentiation of amino acid exchanges are indicated above and to the right of the CP ORF. The AgeI site is unique to CRSV 1.26. The detection of virions in the inoculated leaves and systemic movement to the upper, noninoculated leaves is displayed to the right of the various RNA-1 constructs.