FIG. 3.

Detection of CRSV virion RNA. Representative agarose gel images are shown for RT-PCRs specific for either CRSV RNA-1 (A) or CRSV RNA-2 (B) from purified virion RNA. Each inoculation involved virion purification and subsequent RNA extraction from inoculated (I) and upper, noninoculated (N) leaves. An amplification control reaction (lane denoted DNA) was also performed on a control viral cDNA template of either CRSV RNA-1 or RNA-2. MW denotes the 100-bp DNA ladder (New England Biolabs, Beverly, Mass.), with the 500-bp fragment marked with an arrow to the right of the image.