TABLE 1.

Immortalization of transfected PBMCs by ACH-envelope clones

Clonea	No. of immortalized culturesb/no. of electroporation expts	p19 expression in immortalized cellsc
ACH.WT	NTd	NT
ACH-HTE	3/5	+
ACH-75	3/4	+
ACH-81	2/5	+
ACH-95	4/5	+
ACH-101	0/5	−
ACH-105	4/5	+
ACH-195	4/5	+
ACH-EN	0/5	−
ACH-CT	0/5	−
pBluescript KS	0/5	−
ACH-101/VSV-G	0/3	−
ACH-EN/VSV-G	0/3	−
ACH-CT/VSV-G	0/3	−

^aClones used for this assay are shown. 

^bDetermined by MTT assays (31). 

^cResults of p19 antigen expression in immortalized cells at 90 days posttransfection, as previously described (33). + and − p19 expression of greater than 4 ng/ml and less than 25 pg/ml, respectively. 

^dNT, not tested.