Figure 1.

Experimental design and setups

(A) Experimental timeline. At P1, transduction of light-gated ChR2 channels in M/TCs was performed by AAV injections into the right OB of Tbet-cre mice. At P8, P9, P10 or P11, acute in vivo electrophysiological recordings were performed in the right OB combined with optogenetic activation of ChR2-expressing M/TCs.

(B) The setup for microinjections on neonatal mice (devices corresponding to the numbers: ①infusion pump; ②stereotaxic apparatus; ③heating pad; ④anesthesia mask; ⑤stereomicroscope; ⑥ice box with viral construct; ⑦microliter syringe).

(C) The setup for in vivo surgery (devices corresponding to the numbers: ⑧hot bead sterilizer; ⑨anesthesia induction box; ⑩anesthesia flow meter; ⑪isoflurane vaporizer; ⑫gas evacuation unit; ⑬isotonic sodium chloride solution; ⑭dental cement powder (left) and aqueous solution (right); ⑮needle; ⑯scissors; ⑰forceps).