Procedure for ChR2 transduction in the OB by virus injection at P1
(A) (i) 3D schematic of the OB position in the brain with the injected area in green (top view).
(B) (i) An anesthetized P1 mouse with injection location for the right OB marked. (ii) A head-fixed P1 mouse in front of the anesthesia mask and the tip of the microliter syringe positioned into the right OB for viral injection.
(C) Pictures showing hChR2-EYFP expression in the right OB (i: top view; ii: lateral view of the brain) from a P8 Tbet-cre mouse, and expression pattern across the OB layers on a coronal brain slice (iii).