FIG. 3.

CTL responses to E7 induced by VacLLOE7 surpass those seen with VacSigE7LAMP-1 and VacE7. Mice were injected twice i.p. 7 days apart with 10⁷ PFU. Thirteen days after the final injection, spleen cells from two mice per treatment group were harvested and pooled as described in Materials and Methods. EL4 cells, EL4 cells pulsed with a peptide from HPV-16 E7 (RAHYNIVTF) (EL4 + E7 peptide), or EL4 cells stably transfected with full-length HPV-16 E7 (EL4E7) were compared with the parental EL4 cell line as targets for lysis. CTL activity is expressed as the average percent specific lysis for measurements at each E:T ratio as described in Materials and Methods. Error bars indicate standard deviations for measurements in triplicate or quadruplicate. Results shown are representative of three independent experiments.