FIG. 5.

VacLLOE7 and VacSigE7LAMP-1 induce similar numbers of tetramer-positive T cells that are specific for E7–H2-D^b and that secrete IFN-γ in response to the HPV-16 peptide RAHYNIVTF. Spleen cells from mice vaccinated twice i.p. with each vaccinia virus construct were harvested 7 days after the last vaccination, incubated with 1 μM HPV-16 E7 (A) or HIV Gag (B) peptide, and stained for surface markers as described in Materials and Methods. Cells were gated on CD8⁺ CD62L^lo, and IFN-γ production by E7–H2-D^b tetramer-positive cells was determined. IFN-γ production in the presence of an irrelevant peptide (AMQMLKETI) from HIV Gag was less than 10% the value shown for each vaccinia virus recombinant expressing E7. Responses shown are representative of two independent experiments.