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IFN-γ secretion by E7–H2-Db-specific tetramer-positive CD8+ cells in the spleens of tumor-bearing mice is indistinguishable between mice receiving different vaccinia virus constructs expressing E7. Spleen cells from vaccinated mice were harvested and stimulated as described in the legend to Fig. 5. Plots are of percentages of activated (CD62Llo) CD8+ cells that secreted IFN-γ in response to the HIV Gag control peptide (A) or RAHYNIVF (B).
