TABLE 1.
Enhanced accumulation of E7-specific CD8+ T cells in TC-1 tumors of mice treated with VacLLOE7 is not influenced by collagenase and DNase treatment of tumorsa
| Treatment | % of CD8+ tetramer-positive cells for the indicated sample in:
|
||||
|---|---|---|---|---|---|
| Expt 1
|
Expt 2
|
||||
| Spleen | Tumor with enzyme treatment | Spleen | Matrigel tumor with enzyme treatment | Matrigel tumor without enzyme treatment | |
| VacGag | 0.05 | 1.07 | 0.23 | 0.2 | 0.01 |
| VacE7 | 0.06 | 3.9 | 0.07 | 4.7 | 7.9 |
| VacSigE7LAMP | 0.04 | 8.6 | 0.19 | 15.6 | 18.3 |
| VacLLOE7 | 0.05 | 15.9 | 0.48 | 18.2 | 25.8 |
a
The percentages of RAHYNIVTF-specific cells in the spleens of treated mice are also shown for comparison. Values shown are percentages of cells that fall within the live lymphocyte gate.