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. 2001 Oct;75(20):9671–9678. doi: 10.1128/JVI.75.20.9671-9678.2001

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Copyright © 2001, American Society for Microbiology

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FIG. 1 — Design of a mutant tRNA and cloning into the retroviral vector, N2A. (A) Schematic diagram depicting the primary and secondary structures of tRNA and design of the A58U mutation. Region marked by curved arrow anneals to the HIV-1 PBS. 1-Methyladenosine-58 is shown with a vertical arrow. (B) A complete tRNAA58U polymerase III transcriptional unit (hatched box) was inserted into the 3′ LTR of the murine leukemia virus-derived retroviral vector, N2A (9), by using a unique SnaBI restriction endonuclease site. S, 5-methoxycarbonylmethyl-2-thiouridine; Ψ, pseudouridine; D, dihydrouridine; Tm, 2′-O-methyl-5-methyluridine; R, 2-methylthio-n-6-threonyl carbomoyladenosine.

Inline graphic — Design of a mutant tRNA and cloning into the retroviral vector, N2A. (A) Schematic diagram depicting the primary and secondary structures of tRNA and design of the A58U mutation. Region marked by curved arrow anneals to the HIV-1 PBS. 1-Methyladenosine-58 is shown with a vertical arrow. (B) A complete tRNAA58U polymerase III transcriptional unit (hatched box) was inserted into the 3′ LTR of the murine leukemia virus-derived retroviral vector, N2A (9), by using a unique SnaBI restriction endonuclease site. S, 5-methoxycarbonylmethyl-2-thiouridine; Ψ, pseudouridine; D, dihydrouridine; Tm, 2′-O-methyl-5-methyluridine; R, 2-methylthio-n-6-threonyl carbomoyladenosine.