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Expression of HIV-1 receptor and coreceptor in mutant tRNA
A58U CEM cells. The presence of the CD4 and CXCR4 molecules on the surface of cells was assessed by flow cytometry. CD4 was detected using a phycoerythrin (PE)-conjugated monoclonal antibody to human CD4 (Caltag), and CXCR4 was detected with PE-conjugated anti-fusin (Pharmingen). E and F denote negative and positive gates, respectively.
