FIG. 6.

Single-step infection of normal and mutant cells with a replication-defective virus. (A) Schematic representation of the components needed for the defective vector, HIV–GFP–ΔEnv/VSV-G. The defective transfer vector, HIV-GFP-ΔEnv, contains the GFP reporter gene in place of nef and is envelope defective. The VSV-G envelope is supplied in trans by the plasmid, HCMV–VSV-G. (B) GFP expression as a measure of infection with HIV–GFP–ΔEnv/VSV-G in normal CEM and CEM cells transfected with tRNAA58U.