FIG. 6.

Comparative expression of nucleotide sequences specific to NF-1 class D or JCV T antigen in KG-1 or PMA-treated KG-1 cells. Cultures of KG-1 or PMA-treated KG-1 cells were transfected with either calf thymus DNA or a plasmid containing the NF-1 class D gene and subsequently adsorbed with JCV. RNA was extracted from these cells, and specific nucleotide sequences for NF-1 class D or JCV T antigen were reverse transcribed and amplified by PCR. (A) Results of expression of the NF-1 class D-specific sequence. Lanes 1 and 2 show the respective results from KG-1 or PMA-treated KG-1 cells transfected with calf thymus DNA and adsorbed with JCV; lane 3 shows the results from PMA-treated KG-1 cells transfected with the NF-1 class D containing plasmid and adsorbed with JCV. (B) Results of expression of the JCV T-antigen (TAg)-specific sequence. The order of the samples is the same as that in panel A.