FIG. 5.

Wild-type and mutant hAPN glycoproteins expressed at similar levels on the plasma membrane are differentially infected by HCoV-229E. Cell lines that expressed similar levels of wt-hAPN (A and B), p6-hAPN (C and D), hAPN/N²⁹¹KT (E and F), hAPN/E²⁹¹KT (G and H), hAPN/N²⁹¹KQ (I and J), or a line created by transfection with pCi-neo (K and L) were inoculated with HCoV-229E at an MOI of 1. hAPN expression was identified by indirect IFA with MAb WM47 (A, C, E, G, I, and K), and HCoV-229E spike glycoprotein expression was identified with anti-spike glycoprotein MAb 511H.6 (B, D, F, H, J, and L).