FIG. 6.

Binding of HCoV-229E was inhibited by the addition of a glycosylation signal at amino acid 291 of hAPN. CMT93 cell lines that had similar levels of hAPN surface expression were mixed with ³H-labeled virus, the cells were washed and lysed, and ³H was counted as described in Materials and Methods. The average ³H-labeled virus bound by the wt-hAPN was 1.38 × 10⁴ cpm and was defined as 100% binding. The average total counts bound by cells transfected with pCi-neo was defined as 0% binding. The average of the other cell lines was reported as a percentage of wild-type binding activity, and the error bars represent the standard deviation of the three replicates.