TABLE 1.
Oligonucleotides used for PCR, mutagenesis, and sequencing
Primer | Sequence (5′-3′) |
---|---|
Forward | |
hAPN-111 | CACCATCACCATGGCCAAGG |
h-pAPN-991 | AACGAGACGGCACAAAATGGTGTCTTG |
hAPN E291N-Q293T/F | GTTCGACTACGTTAACAAGACGGCATCCAATGG |
m-hAPN N291E/F | TTCGACTACGTTGAGAAGACGGCATCC |
m-hAPN T293Q/F | TACGTTAACAAGCAGGCTAGCAATGGTGTCTTG |
hpAPN-N291E/F | CAATCCGTTGAGGAGACGGCACAA |
hpAPN-T293Q/F | TCCGTTAATGAGCAGGCACAAAATGGT |
hAPN-N818K/F | GAGCAGTTCCGGAAAGCCACACTGGTC |
hAPN-T820E/F | CCGAAATGCCGAGCTCGTCAATGAGGC |
DT-021 | CCCACTTCCAGAAGACCCC |
Reverse | |
hAPN-990R | AACGGATTGGAACTCACTGACAATGAA |
DT-022 | AGTTCTCCCGGTAGGTCACC |
hAPN E291N-Q293T/R | CCATTGGATGCCGTCTTGTTAACGTAGTCGAAC |
m-hAPN N291E/R | GGATGCCGTCTTCTCAACGTAGTC |
m-hAPN T293Q/R | CAAGACACCATTGCTAGCCTGCTTGTTAACGTA |
hpAPN-N291E/R | TTGTGCCGTCTCCTCAACGGATTG |
hpAPN-T293Q/R | ACCATTTTGTGCCTGCTCATTAACGGA |
hAPN-N818K/R | GACCAGTGTGGCTTTCCGGAACTGCTC |
hAPN-T820E/R | GCCTCATTGACGAGCTCGGCATTTCGG |