FIG. 7.

Anti-γ-tubulin and anticentrin immunofluorescence. HDFs were plated on coverslips in the presence of HU for 24 h and then mock infected or infected with Ad-ST at 20 PFU/cell for 8 h. Cell cycle progression was then initiated by washing out the HU and adding DME–10% FCS. At various times after HU release, cells were fixed and permeabilized and then stained for γ-tubulin. The cells shown were stained with anti-γ-tubulin (A) 10 h after HU release or with anticentrin antibody (B) 15 h after HU washout. Cells infected with Ad-ST are shown on the right side of each panel, and control Ad-CMV-infected cells are on the left.