FIG. 4.

Disruption of the dynein-dynactin complex results in inhibition of infection. (a, c, d, and e) A plasmid expressing Myc-tagged p50/dynamitin was transfected into Vero cells and detected using antibodies against the Myc epitope tag and secondary anti-mouse Ig-rhodamine (in red). (b, c, d, e) Cells were infected with ASFV and showed characteristic morphological changes. Virus proteins were detected with antibodies against p30 (c), anti-p54 (b and d), and anti-p72 primary antibodies (e) labeled with Alexa 488 (in green). Cells transfected with dynein dominant-negative mutant were not infected, and no simultaneous expression of both viral proteins and p50/dynamitin was found in the same cell. Percentages of infected cells were over 95% in control infected but nontransfected cultures, as shown in panel b. Bar, 10 μm.