FIG. 1.
Similar pattern of antigen recognition among T-cell clones expressing TCR BV13S1. (A) Antigen dose-response cytotoxic activity of Tax11-19-reactive CD8+ T-cell clones. HLA-A*0201-expressing EBV-transformed B-cell lines were pulsed with the indicated concentrations of Tax11-19 peptide and used as target cells in a 4-h 51Cr-release assay. The effector-to-target ratio was 10:1. (B) Specific binding of HLA-A*0201/Tax11-19 multimer by CD8+ T-cell clones expressing TCRBV13S1. T-cell clones were incubated with the indicated dilutions of tetramer at 4°C for 1 h. The left y axis indicates the mean fluorescence intensity of multimer binding, while the right y axis shows the mean fluorescence intensity of TCR expression.