FIG. 4.

Different recognition patterns of CD8⁺ T-cell clones toward naturally occurring peptides. Differential recognition of natural peptides by CD8⁺ Tax11-19-reactive T-cell clones expressing BV13S1 is shown. Human and microbial peptides expressing the recognition motif for B7 TCR were previously published (12) and used in this study. Recognition patterns of CD8⁺ T-cell clones were determined by a ⁵¹Cr release assay at a peptide concentration of 10 μM. Of 16 peptides tested, 3 were recognized by CD8⁺ T-cell clones, and reactivity toward these 3 peptides is shown. As previously published (12), the B7 clone recognized the above peptides with a range of 18 to 20% specific lysis. a.a., amino acid.