TABLE 1.

TCR alpha and beta amino acid sequences of Tax11-19-specific T cells isolated and cloned by the use of HLA-A^*0201/Tax11-19 tetramers

Clonea	TCR	Sequenceb	J
		VN/Junctional region
Alpha region
TP 35	TCRAV1S1	Y F C A T V R D     G N N T D K L I F G T G T R Q V F P	34
TP 33, 80	TCRAV2	Y Y C A V     A H N Y G Q N F V F G P G T R L S V L	26
TP 21	TCRAV3S1	Y F C A V       E Q R M V A D K L I F G T G T R L Q V F P	34
TP 20, 32, 41, 58, 63	TCRAV4S1	Y Y Y C I     R S G S A R Q L T F G S G T Q L T V L P	22
TP 43	TCRAV12S2	Y L C A V     D S G G Y Q K V T F G I G T K L Q V I P	13
TP 79	TCRAV12S2	Y L C A V N     I G F G N V L H C G S G T Q V I V L P	35
TP 27	TCRAV12S2	Y L C A V N   P P F G N E K L T F G T G T R L T I I P	48
TP 7	TCRAV14S1	Y F C A M     M G G G S E K L V F G K G T K L T V N P Y	57
TP 40	TCRAV14S1	Y F C       A W D N A G N M L T F G G G T R L M V K P H	39
TP 26, 45	TCRAV14S1	Y F C       A Y L N T G N Q F Y F G T G T S L T V I P	49
TP 60	TCRAV14S1	Y F C A Y R T L Y D N F G N E K L T F G T G T R L T I I P	48
TP 61	TCRAV15S1	Y I C A V K E S L V E M R K L T F G T G T R L L T I I P	48
TP 34	TCRAV17S1	Y F C       A C F Q G A Q K L V F G Q G T R L T I N P	54
TP 20, 32, 41, 58, 63, 61, 71, 72, 73, 10, 59	TCRAV17S1	Y F C       A A F Q G A Q K L V F G Q G T R L T I N P	54
Beta region
TP 45	TCRBV7	Y L C A S S Q E R L S F G T K N I Q Y F G A G T R L S V L	2.4
TP 79	TCRBV8	Y F C A S S     S E Y R S G A N V L T F G A G S R L T V L	2.6
TP 26, 60	TCRBV8	Y F C A S S     K P S V M N T E A F F G Q G T R L T V V	1.1
TP 20, 32, 41, 58, 63 (I)	TCRBV13S1	Y F C A S S       V P G A G E E T Q Y F G P G T R L L V L	2.5
TP10, 73 (II)	TCRBV13S1	Y F C A S S       S P G Q G N Y E Q Y F G P G T R L T V T	2.7
TP 34 (III)	TCRBV13S1	Y F C A S S       T P G Q G A Y E Q Y F G P G T R L T V T	2.7
TP 61 (IV)	TCRBV13S1	Y F C A S S       Y P G Q G E H E Q Y F G P G T R L T V T	2.7
TP 59, 71, 72 (V)	TCRBV13S1	Y F C A S S       S P G T G V N E Q F F G P G T R L T V L	2.1
TP 43	TCRBV13S1	Y F C A S S     K P G L G G T G E L F F G E G S R L T V L	2.1
TP 48	TCRBV13S1	Y F C A S S     R P G L A G R N E Q F F G P G T R L T V L	2.1
TP 27	TCRBV16S1	Y F C A S     Q L G Q G V H G Y G Y T F G S G T R L T I V	1.2
TP 33, 80	TCRBV16S1	Y F C A S   Q D F L S S G A Y N E Q F F G P G T R L T V L	2.1
TP 7	TCRBV21S1	Y L C A S S           L T I G G A Q F F G Q G T R L T V V	1.1
TP 21	TCRBV21S1	Y L C A S S             F M T D T Q Y F G P G T R L T V L	2.3
TP 35	TCRBV21S1	Y L C A S S               L P D T Q Y F G P G T R L T V L	2.3
TP 40	TCRAV17S1	Y L C A S S           A R L G Q P Q H F G D G T R L S I L	1.5

^aT-cell clones expressing TCRBV13S1 were divided into groups (I to Y, indicated in parentheses) depending on the CDR3 region sequence. 

^bN or N(D)J additions for both alpha and beta chains are indicated in bold.