Fig. 6. NRL alters the stoichiometry of CRX binding to Ret4 site.

SEC-MALS profile of the complex of CRX HD and NRL DBD with Ret4-NRE DNA. Refractive index (black) curve is shown together with the molar mass (red) calculated by MALS. The molar mass of the major peak (~60 kDa) corresponds well to the predicted mass of the DNA-bound complex with the NRL dimer (2 molecules) and one molecule of CRX HD.