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. Author manuscript; available in PMC: 2025 Oct 3.
Published in final edited form as: Structure. 2024 Jul 30;32(10):1751–1759.e4. doi: 10.1016/j.str.2024.07.004

Fig. 7. NRL modulates CRX binding affinity to the rhodopsin proximal promoter.

Fig. 7.

A. Representative raw and idealized traces from smTIRF experiments where Cy3-labelled CRX HD binds to a surface tethered Ret4-NRE DNA molecule. B, C, D, The dwell-time distributions were constructed from all “ON” dwell times for Ret3-NRE DNA binding of 1 nM of CRX HD alone (B), or in the presence of 2.5 nM (C) and 5 nM of NRL DBD (D). The dissociation rate constants (koff) from a single-exponential fit of the distributions were (s−1): B, 0.303± 0.011; C, 0.218 ± 0.024 D. 0.154 ± 0.013.