. Author manuscript; available in PMC: 2025 Oct 3.

Published in final edited form as: Structure. 2024 Jul 30;32(10):1751–1759.e4. doi: 10.1016/j.str.2024.07.004

Table 1.

Single-molecule TIRF microscopy summary

S.No.	Complex (Protein-DNA)	DNA	No. of traces taken	No. of Events (On Events)	State 1 (v_on) per sec	State 1 (k_on) s ⁻¹ M⁻¹	State 2 (k_{off or} ) s⁻¹	State 2 (tau) s	smTIRF Kd=k_off/k_on
1.	1 nM CRX	500 pM	146	285	0.01	0.01 s⁻¹ nM	0.303 ± 0.011	2.286	55.1 nM
2.	1 nM CRX 2.5 nM NRL	500 pM	101	198	0.01	0.01 s⁻¹ nM	0.218 ± 0.024	4.854	39.63 nM
3.	1 nM CRX 5 nM NRL	500 pM	124	215	0.01	0.01 s⁻¹ nM	0.154 ± 0.013	6.513	28 nM