|
Bacterial and virus strains
|
|
E.coli BL21-AI |
Invitrogen |
Cat # C6070-03 |
|
Chemicals, peptides, and recombinant proteins
|
| sulfo-Cyanine3 maleimide |
Lumiprobe |
Cat # 21380 |
| Biotin-PEG6-Maleimide |
Thermo Fisher Scientific |
Cat # B556350MG |
| Octet Streptavidin (SA) Biosensor |
Sartorius |
Item # 18-5019 |
| Trolox |
Sigma-Aldrich |
Cat # 238813-5G |
|
Deposited data
|
| Crystal structure of the CRX HD bound to Ret4 response element. |
This study |
PDB 9B8U |
| CRX HD-Ret4 oligonucleotide complex SAXS data |
This study |
SASBDB: SASDU29 |
|
Oligonucleotides
|
| RET4.f: CCAGGAGCTTAGGAGGGGGAG |
This study |
N/A |
| RET4.r: CCTCCCCCTCCTAAGCTCCTG |
This study |
N/A |
| NRE-RET4.f: CCAGATGCTGATTCAGCCAGGAGCTTAGGAGGGGGAGG |
This study |
N/A |
| NRE-RET4.r: CCTCCCCCTCCTAAGCTCCTGGCTGAATCAGCATCTGG |
This study |
N/A |
| CRX_R41Q.f: AAGCAGCGGCAGGAGCGCACCACCTTCACC |
This study |
N/A |
| CRX_R41Q.r: GGTGCGCTCCTGCCGCTGCTTCCTGGGGGC |
This study |
N/A |
| CRX_E42K.f: CAGCGGCGGAAACGCACCACCTTCACCCGG |
This study |
N/A |
| CRX_E42K.r: GGTGGTGCGTTTCCGCCGCTGCTTCCTGGG |
This study |
N/A |
| CRX_R90W.f: TTCAAGAACTGGAGGGCTAAATGCAGGCAG |
This study |
N/A |
| CRX_R90W.r: TTTAGCCCTCCAGTTCTTGAACCAAACCTG |
This study |
N/A |
|
Recombinant DNA
|
| CRX HD in pET-21a |
This study |
N/A |
| NRL DBD in pET-21a |
This study |
N/A |
|
Software and algorithms
|
| BioXTAS RAW 2.1.1 |
Hopkins et al 61
|
https://bioxtas-raw.readthedocs.io/en/latest/
|
| COOT |
Emsley et al 62
|
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
|
| PHENIX |
Liebschner et al 63
|
https://phenix-online.org/
|
|
Other
|
| His-Bind resin |
Novagen |
Cat #69670 |
| HiLoad 16/600 Superdex 75 column |
GE Healthcare |
Cat #28989333 |
| SP sepharose fast flow resin |
GE Healthcare |
Cat #17072901 |
| Catalase |
Sigma-Aldrich |
Cat # C40-500MG |
| Glucose oxidase |
Sigma-Aldrich |
Cat # G2133-50KU |
| NeutrAvidin |
Thermo Fisher Scientific |
Cat # 31000 |
