KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-septin-7 | Thermo Fisher Scientific | Cat# PA5-56181; Cat# MM-0070-2-P ; RRID:AB_2647129 |
| mouse anti-C4F6 SOD1 antibody | MediMabs | RRID:AB_10015296 |
| Alexa Fluor 488 goat anti-rabbit | Thermo Fisher Scientific | Cat# R37116; RRID:AB_2556544 |
| Alexa Fluor 635 goat anti-mouse | Thermo Fisher Scientific | Cat# A-31574; RRID:AB_2536184 |
| Hoechst 33342 | Invitrogen | Cat# H3570; RRID:AB_10626776 |
| Anti-SOD1 | Sigma | Cat# SAB5200083; RRID:AB_2890259 |
| secondary antibody conjugated to HRP | Thermo Fisher Scientific | Cat# A16096; RRID:AB_2534770 |
| Chemicals, peptides, and recombinant proteins | ||
| carboxyl magnetic beads (AbraMag™) | AbraMag | C859T34 |
| septin-7 | LSBio | LS-G23386-100 |
| Critical commercial assays | ||
| iTRAQ mass spectrometry labeling | Sigma | 4381662-1KT |
| Monolith His-Tag labeling kit (RED-tris-NTA 2nd generation) | NanoTemper | SKU: MO-L018 |
| Deposited data | ||
| Mass spectrometry proteomics data | This paper | ProteomXchange Consortium via the PRIDE partner repository: dataset identifier PXD050873 |
| The RNA-seq data have been deposited to the Gene Expression Omnibus (GEO) titled “RNA-seq of NSC-34 cells expressing SOD1.” | This paper | GSE263048 |
| All other data is published in Mendeley Dataset under citation | This paper | Choi, Esther (2024), “Superoxide dismutase 1 (SOD1) trimer interactome from mouse brain, spinal cord, and skeletal muscle tissue”, Mendeley Data, V1, doi: 10.17632/8829xvpgy y.1 |
| Septin-7 | Brognara et al.99 | PDB ID: 6N0B |
| Septin-7-2G/Septin-6/Septin-7 heter-hexamer | Mendonça et al.101 | PDB ID 7M6J |
| Experimental models: Cell lines | ||
| NSC-34 cells | Cedarlane Labs | RRID:CVCL_D356 |
| Experimental models: Organisms/strains | ||
| Wild-type black CD-1 male mice | Penn State Animal Research Facility (ARF) | RRID:IMSR_CRL:02 2 |
| Oligonucleotides | ||
| Primer: A4V Forward: GGCGACGAAGgtCGTGTGCGTG Reverse: ATGTTATTGGGCGATCCCAATTACACCAC | This paper | N/A |
| Primer: F20L Forward: ATCATCAATTtagAGCAGAAGGAAAGTAATGGACCAG TGAAG Reverse: D101I GCCCTGCACTGGGCCGTC | This paper | N/A |
| Primer: H46Q Forward: GCATGGATTCcagGTTCATGAGTTTG Reverse: AGGCCTTCAGTCAGTCCT | This paper | N/A |
| Recombinant DNA | ||
| Dox-XLone plasmid | Addgene | Plasmid #96930 |
| Software and algorithms | ||
| Gaia | Kota et al.100 | https://dokhlab.med.psu.edu/chiron/login.php |
| Chiron | Ramachandran et al.102 | http://chiron.dokhlab.org |
| ClusPro 2.0 | Kozakov et al.103 | https://cluspro.org/help.php |
| DESeq2 (2_1.6.3) | Wang et al.96 | https://bioconductor.org/packages/release/bioc/html/DESeq2.html |