Key resource table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and virus strains | ||
| DH5a Competent Cells (E. coli) | NEB | C2987H |
| BL21(DE3) Competent Cells (E.coli) | NEB | C2527 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Luria Broth | RPI | L24400-5000.0 |
| IPTG | Chem Impex | 00194-100g |
| Ampicillin | Fisher Scientific | BP1760-25 |
| Carbenicillin | Gold Biotechnology | 50-153-2751 |
| Chloramphenicol | Fisher Scientific | AC227921000 |
| L-(+)-Arabinose | Sigma Aldrich | A3256-100G |
| Chaperone Plasmid Set | Takara Biotech | 3340 |
| Sodium Chloride | RPI | S23025-3000.0 |
| Tris Base Ultra-Pure | RPI | T60040-5000.0 |
| HEPES | RPI | H75030-1000.0 |
| Imidazole | Fisher Scientific | AAA102210E |
| DTT | RPI | D11000-50.0 |
| TCEP | Thermo Scientific | PI20491 |
| NP-40 | Sigma Aldrich | I3021-100ML |
| Fluorescein-5-maleimide | Fisher Scientific | 50-187-4590 |
| DMAB | Acros Organics | AC177310250 |
| Paraformadehyde | Thermo Scientific | 28906 |
| Magnesium Chloride | RPI | M24000-500.0 |
| Adenosine-5-triphosphate disodium salt | Chem Impex | 00015-25G |
| Adenosine-5-diphosphate disodium salt | RPI | A11220-10.0 |
| Adenylyl-imidodiphosphate salt | Sigma-Aldrich | SIAL-10102547001 |
| Polyethylene-glycol-3350 | Hampton Research | HR2-527 |
| 4-15% Mini Protean Stain free protein gels | Bio-Rad | 4568086 |
| Coomassie Brilliant Blue- R250 | Thermo Scientific | 20278 |
| 4X SDS-Sample buffer | Fisher Scientific | BP-110R |
| Glycine | RPI | G36050-5000.0 |
| Acetic acid | RPI | 50-197-7981 |
| SDS | Fisher Scientific | BP166-500 |
| SYPRO Orange | Thermo Scientific | S6650 |
| Pro-Q Diamond Phosphoprotein Gel Stain | Invitrogen | P33301 |
| Lysozyme | Hampton Research | HR7-110 |
| D-Ribose | Fischer Scientific | AAA1789409 |
| Critical Commercial Assays | ||
| Transcreener-ADP2 Fp assay | Sigma-Aldrich | 3010-1K |
| ADP-Glo Assay | Promega | V6930 |
| QIAprep Spin Miniprep Kit | Qiagen | 27106 |
| QIAquick PCR & Gel Cleanup Kit | Qiagen | 28506 |
| Deposited Data | ||
| HsFN3K Crystal structure | This paper | PDB: 8UE1 |
| Recombinant DNA | ||
| Plasmid pMBP-His6TEV | This paper | N/A |
| pMBP-His6TEV-FN3K | This paper | N/A |
| pMBP-His6TEV-FN3K Δ116-138 | This paper | N/A |
| pMBP-His6TEV-FN3K Δ147-156 | This paper | N/A |
| pMBP-His6TEV- FN3K SWAP1 | This paper | N/A |
| pMBP-His6TEV- FN3K SWAP2 | This paper | N/A |
| pMBP-His6TEV- FN3K F39A | This paper | N/A |
| pMBP-His6TEV- FN3K K41A | This paper | N/A |
| pMBP-His6TEV- FN3K E55A | This paper | N/A |
| pMBP-His6TEV- FN3K D217A | This paper | N/A |
| pMBP-His6TEV- FN3K D217E | This paper | N/A |
| pMBP-His6TEV- FN3K D217Q | This paper | N/A |
| pMBP-His6TEV- FN3K D217N | This paper | N/A |
| pMBP-His6TEV- FN3K W219A | This paper | N/A |
| pMBP-His6TEV- FN3K N222A | This paper | N/A |
| pMBP-His6TEV- FN3K D234A | This paper | N/A |
| pMBP-His6TEV- FN3K D234E | This paper | N/A |
| pMBP-His6TEV- FN3K D234Q | This paper | N/A |
| pMBP-His6TEV- FN3K D234N | This paper | N/A |
| pMBP-His6TEV- FN3K H288A | This paper | N/A |
| pMBP-His6TEV- FN3K H291A | This paper | N/A |
| Software and Algorithms | ||
| Discover MP 2.4.2 | Refeyn | https://www.refeyn.com/software-release-updates |
| Pymol v2.0.7 | 57 | http://www.pymol.org/ |
| Phyre2 | 58 | http://www.sbg.bio.ic.ac.uk/~phyre2/ |
| PHENIX | 59 | https://www.phenix-online.org/ |
| Coot | 60 | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
| PHASER | 61 | http://www.ccp4.ac.uk/html/phaser.html |
| WebLogo 3 | 62 | https://weblogo.berkeley.edu/logo.cgi |
| HKL-3000 | 63 | http://www.hkl-xray.com/ |
| Prism | Prism | https://www.graphpad.com/scientific-software/prism/ |
| Other | ||
| Mass Photometry | Refeyn | N/A |
| EnVision 2102 Multilabel microplate reader | Perkin Elmer | 2102-0010 |
| BioTek Synergy microplate reader | Agilent Technologies | 11-120-533 |
| StepOne Real Time PCR System | Applied Biosystems | 4376600 |
| AKTA Pure Chromatography system | Cytiva Life sciences | 29018224 |
| Monolith NT.115- Microscale thermophoresis | Nanotemper | G008 |
| 384-well Black round bottom microplate | Corning | 4514 |
| 384-well White round bottom microplate | Corning | 4512 |
| 96-well plates | ThermoFisher Scientific | 4346907 |
| HisTrap FF, His-tagged protein purification column | Cytiva Life sciences | 17-5255-01 |
| HiTrap Q HP anion exchange column | Cytiva Life sciences | 17115401 |
| HiTrap S HP cation exchange column | Cytiva Life sciences | 17115101 |
| Superdex™ 200 Increase 10/300 | Cytiva Life sciences | 28-9909-44 |