Fig. 2.

Levels of ER-stress protein GRP78 are elevated in brains of mice that underwent MI vs. sham-MI. A Immunohistochemistry (IHC) of cortical and hippocampal brain regions from sham-MI mice (“Con”) and MI mice, 7 days after LCA ligation or sham/control surgery (each N = 5). B Combined quantitation of both cortex and hippocampal IHC shows significant upregulation of GRP78 (~ 30%) in these brain regions after MI relative to sham-MI (**P < 0.005). C Western blot of total protein from sham-MI, MI, and EMI mouse brains, probed with antibodies to GRP78 and β-actin (each N = 3). D Quantitation of the blot shows GRP78/β-actin expression was elevated in mouse brain after MI relative to sham-MI (N = 6; P ≈ 0.06) and relative to EMI (P ≈ 0.05) by 2-tailed heteroscedastic t tests