FIG. 2.
Dose-dependent baculovirus-mediated gene transfer into primary rat hepatocytes in long-term DMSO culture. (A) Fifty-five days postseeding, primary rat hepatocytes in long-term DMSO culture were infected with 3, 6, 12, 25, 50, or 100 PFU of CMV-lacZ baculovirus/cell. At 24 h p.i., cultures were stained for β-Gal activity and the percent positive peripheral (edge) cells was determined (□). In a separate experiment, primary rat hepatocytes in long-term DMSO culture for 56 days were infected with 50, 100, 200, 400, or 800 PFU of CMV-lacZ baculovirus/cell (●). At 24 h p.i., cultures were stained for β-Gal activity. Results show the percentage of peripheral hepatocytes positive for β-Gal activity, with the standard deviation, at the indicated PFU of CMV-lacZ baculovirus/cell. (B) Primary rat hepatocytes in long-term DMSO culture for 56 days were mock infected and stained for β-Gal activity 24 h later. (C) Primary hepatocytes in long-term DMSO culture for 56 days were infected with 800 PFU of CMV-lacZ baculovirus/cell and stained for β-Gal activity at 24 h p.i.