FIG. 6.
Effect of calcium depletion on baculovirus-mediated gene delivery to internal hepatocytes. At day 20 postseeding, primary rat hepatocytes were pretreated in either control medium, calcium-free DMEM, or calcium-free DMEM supplemented with either 25 or 100 μM EGTA for 1 h. Subsequently, cultures were infected for 1 h with 400 PFU of CMV-lacZ baculovirus/cell diluted in either control medium (A), calcium-free DMEM (B), or calcium-free DMEM supplemented with either 25 (C) or 100 (D) μM EGTA. At 24 h p.i., cultures were stained for β-Gal activity.