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Elution profiles of viral IE proteins from Sepharose CL-2B gel filtration chromatography. Nuclear extracts prepared from cells infected with wild-type HSV-1 were chromatographed on Sepharose CL-2B columns, and fractions were analyzed by immunoblotting. Blots were probed with antibodies that recognize ICP4, ICP0, ICP22, and ICP27 as indicated. Relative densitometry units were determined and plotted as described for Fig. 3. Positions of 2-MDa and 670-kDa molecular standards are indicated.
