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β-Galactosidase activity of U87-MGneor clones 1 through 16
| Hybridizing probe(s)a | No. of clones | No. of clones with β-galactosidase activityb
|
|||
|---|---|---|---|---|---|
| − | + | ++ | +++ | ||
| None | 1 | 1 | |||
| Ψ | 5 | 3 | 1 | 1 | |
| SV40pA | 2 | 1 | 1 | ||
| Ψ + SV40pA | 8 | 2 | 3 | 3 | |
As determined by Southern blot analysis (Fig. 5). Ψ, probe for Ad5 packaging signal (5′ end of MVX-lacZneo).
β-Galactosidase activity was determined by the percentage of cells turning blue after X-Gal staining (−, 0%; +, <10%; ++, 10 to 50%; +++, 50 to 100%).
