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. Author manuscript; available in PMC: 2024 Oct 6.
Published in final edited form as: Nat Aging. 2023 Dec 8;4(1):95–109. doi: 10.1038/s43587-023-00538-3

Extended Data Fig. 4 |. Validation of reprogrammed neurons of rescuing or non-rescuing condition for ATAC-sequencing.

Extended Data Fig. 4 |

a, RCAN1 expression in fibroblasts transduced with shRCAN1 (top) or RCAN1 (middle) in a dose-dependent manner. RCAN1 expression in HD-MSNs (HD.43) transduced with shCtrl, shRCAN1, or RCAN1 (bottom). b, Representative image (top) and quantification (bottom) of DARPP-32-positive cells from four independent HD-MSNs transduced with shCtrl, shRCAN1, or shCaN (HD.43, HD.40, HD.47, HD.45). Cells were immunostained with anti-DARPP-32 and TUBB3 antibodies. An average of 183 cells of each were counted from three or more randomly chosen fields (n = 4). Scale bars represent 10 μM. c, RT-qPCR analysis of the expression of RCAN1 and CaN in (b) (n = 12, 12, 8, 8). Statistical significance was determined using one-way ANOVA with Tukey’s post-hoc test (b) and two-tailed unpaired t-test (c); ****p < 0.0001, ns, not significant, and mean±s.e.m (b,c). The sample size (n) corresponds to the number of biologically independent samples (b,c).