Fig. 3.

Histopathological sections of Besnoitia sp. cysts in muscles stained with hematoxylin and eosin. Cysts with bradyzoites (c) in skeletal muscles (m) (A). Detail of a cyst wall in the heart muscle (m), composed of three layers: capsule (star), host cell cytoplasm (short arrow), and parasitophorous vacuole (long arrow) with a myriad of bradyzoites (br). Note the compressed nucleus of the host cell (N) (B). Higher magnification of a Besnoitia sp. cyst (c) in skeletal muscle (m) showing absence of tissue reaction (C). Degenerated Besnoitia sp. cyst on the tongue (to) surrounded by severe inflammation (short arrow) (D).