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. 2024 Sep 23;43:114–128. doi: 10.1016/j.bioactmat.2024.09.007

Table 2.

Primer sequences used for quantitative RT-PCR analysis.

Gene name Forward primer sequence (5′–3′) Reverse primer sequence (5′–3′) Amplicon length (bp)
GAPDH GACAGTCAGCCGCATCTTCTT TCCGTTGACTCCGACCTTCA 86
ALP GGAACTCCTGACCCTTGACC TCCTGTTCAGCTCGTACTGC 86
RUNX2 GTAGATGGACCTCGGGAACC GAGGCGGTCAGAGAACAAAC 78
COL1A1 GAGTGCTGTCCCGTCTGC TTTCTTGGTCGGTGGGTG 52
OCN GGCAGCGAGGTAGTGAAGAG CTCACACACCTCCCTCCTG 102