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. 2001 Oct;75(20):9977–9982. doi: 10.1128/JVI.75.20.9977-9982.2001

FIG. 1.

FIG. 1

FIG. 1

(A) Detection of DNA from the EBV plasmid and transfected EBER DNA in Akata-EC cell clones. Akata-EC cells were transfected with the EBER plasmid carrying the neomycin resistance gene or a Neor plasmid (as a control) and cultured in selective medium containing 1.5 mg of G418 per ml. G418-resistant cell clones were isolated and continuously cultivated for 1 year. Cellular DNA (10 μg) was isolated from each cell clone at 3 months and 1 year of culture, digested with EcoRI restriction endonuclease, blotted onto a nylon membrane, and hybridized with a 32P-labeled EBER probe. The 3.0-kbp band is DNA from the EBV plasmid, and the 1.1-kbp band is transfected EBER DNA. Photos of ethidium bromide-stained agarose gel are also shown. (B) EBNA expression in EBER- and Neor-transfected Akata EC cell clones. EBNA was stained in the anticomplement immunofluorescence assay.