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. 2024 Aug 22;14(10):jkae203. doi: 10.1093/g3journal/jkae203

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© The Author(s) 2024. Published by Oxford University Press on behalf of The Genetics Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — An E2F-regulated accessible enhancer remains activatable after 24 h APF. Wings (a–c) and eyes (d–f) were dissected from staged pupa and stained for E2F transcriptional activity (anti-GFP for PCNA-GFP reporter) at the time points indicated. Animals were collected as white pre-pupa (0 h APF), staged to a postmitotic stage of 24–28 h APF and heat-shocked for 20 min. to induce Gal4 expression driving E2F (UAS-E2F1 + UAS-DP) or CycD + E2F (UAS-Cyclin D + UAS-Cdk4 + UAS-E2F1 + UAS-DP) postmitotically. Tissues were collected and stained at 40–44 h APF. UAS-RFP shown in magenta, PCNA-GFP shown in green for overlays, and white in single channel a′–f′ panels. Sample numbers; control wings n = 2, E2F expressing wings n = 4, E2F + DK4 expressing wings n = 4, control eyes n = 2, E2F expressing eyes n = 4, E2F + DK4 expressing eyes n = 4.