Skip to main content
. Author manuscript; available in PMC: 2024 Nov 1.
Published in final edited form as: J Mol Biol. 2023 Sep 18;435(21):168283. doi: 10.1016/j.jmb.2023.168283

Figure 2. Isolation of the tripartite Ssq1*-Hsc20-Isu1 and bipartite Hsc20-Isu1 complexes from E. coli co-expressing their constituents.

Figure 2.

(left) Size exclusion chromatography of Ni-NTA purified complexes; peak A, tripartite and peak B, bipartite. For identification of proteins in peak A and B see Figure S7.

(middle, right) Deconvoluted native mass spectrometry (MS) spectra of the tripartite (middle) and bipartite (right) complexes- predicted molecular weights are indicated. For the original native MS spectra see FigureS7.