. Author manuscript; available in PMC: 2024 Nov 1.

Published in final edited form as: J Mol Biol. 2023 Sep 18;435(21):168283. doi: 10.1016/j.jmb.2023.168283

Table 1.

Protein variants used that have disturbed interaction between tripartite complex subunits

Protein	Variant	Location	Affected interaction
Ssq1	F462S	substrate binding cleft	Isu1 (Ref. 34)

Hsc20	R37A	J-domain helix II	Ssq1 (Ref. 32)
	H48A	J-domain HPD	Ssq1 (Ref. 31)
	D50A	J-domain HPD	Ssq1 (Ref. 31)
	LLY (L105A, L109A, Y163A)	CTD	Isu1 (Ref. 23)
	K132A	CTD	Ssq1 (this manuscript)
	K172A	CTD	Ssq1 (this manuscript)
	KK (K132A, K172A)	CTD	Ssq1 (this manuscript)

Isu1	PVK (P135A, V136A, K137A)	LPPVK (Ssq1 substrate)	Ssq1 (Ref. 29)